Laminin alpha 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89955

Novus Biologicals, part of Bio-Techne
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NBP1-89955
NBP1-89955-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin alpha 3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLNRIRTWQKTHQGENNGLANSIRDSLNEYEAKLSDLRARLQEAAAQAKQANGLNQENERALGAIQRQVKEINSLQSDFTKYLTTADSSLLQTNIALQLMEKSQKEYE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22892598)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Laminin alpha 3 Antibody

Immunocytochemistry/ Immunofluorescence: Laminin alpha 3 Antibody [NBP1-89955]

Immunocytochemistry/ Immunofluorescence: Laminin alpha 3 Antibody [NBP1-89955]

Immunocytochemistry/Immunofluorescence: Laminin alpha 3 Antibody [NBP1-89955] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP1-89955]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP1-89955]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP1-89955] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for Laminin alpha 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF: Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 3

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. (provided by RefSeq)

Alternate Names

BM600, BM600 150kD subunit, BM600-150kDa, E170, epiligrin, Epiligrin 170 kDa subunit, epiligrin alpha 3 subunit, Epiligrin subunit alpha, kalinin 165kD subunit, Kalinin subunit alpha, kalinin-165kDa, lama3a, laminin subunit alpha-3, laminin, alpha 3, laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin), laminin-5 alpha 3 chain, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, LAMNA, LOCS, nicein 150kD subunit, Nicein subunit alpha, nicein-150kDa

Gene Symbol

LAMA3

Additional Laminin alpha 3 Products

Product Documents for Laminin alpha 3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin alpha 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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