Laminin alpha 5 Antibody (2F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00003911-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin alpha 5 Antibodies »

Product Specifications

Immunogen

LAMA5 (AAH03355.1, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGVSLRDKKVHWVYQLGEAGPAVLSIDEDIGEQFAAVSLDRTLQFGHMSVTVERQMIQETKGDTVAPGAEGLLNLRPDDFVFYVGGYPSTFTPPPLLRFP

Localization

Secreted, extracellular space, extracellular matrix, basement membrane.

Specificity

LAMA5 - laminin, alpha 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Laminin alpha 5 Antibody (2F7)

Western Blot: Laminin alpha 5 Antibody (2F7) [H00003911-M01]

Western Blot: Laminin alpha 5 Antibody (2F7) [H00003911-M01]

Western Blot: Laminin alpha 5 Antibody (2F7) [H00003911-M01] - Analysis of LAMA5 expression in transfected 293T cell line by LAMA5 monoclonal antibody (M01), clone 2F7.Lane 1: LAMA5 transfected lysate(74 KDa).Lane 2: Non-transfected lysate.
ELISA: Laminin alpha 5 Antibody (2F7) [H00003911-M01]

ELISA: Laminin alpha 5 Antibody (2F7) [H00003911-M01]

ELISA: Laminin alpha 5 Antibody (2F7) [H00003911-M01] - Detection limit for recombinant GST tagged LAMA5 is approximately 0.03ng/ml as a capture antibody.

Applications for Laminin alpha 5 Antibody (2F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 31091437). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 21195710).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Laminin alpha 5

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Alternate Names

KIAA0533, KIAA1907, laminin alpha-5 chain, laminin subunit alpha-5, laminin, alpha 5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

Entrez Gene IDs

3911 (Human)

Gene Symbol

LAMA5

OMIM

601033 (Human)

Additional Laminin alpha 5 Products

Product Documents for Laminin alpha 5 Antibody (2F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin alpha 5 Antibody (2F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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