Laminin Antibody (CL2968)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42384

Novus Biologicals, part of Bio-Techne
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NBP2-42384
NBP2-42384-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2968

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin Antibodies »

Product Summary for Laminin Antibody (CL2968)

Immunogen

Laminin Antibody (CL2968) was made to a recombinant protein corresponding to amino acids: LIVQGRGLIDAAAAQTDAVQDALEHLEDHQDKLLLWSAKIRHHIDDLVMHMSQRNAVDLVYRAEDHATEFQRLADVLYSGLENIRNVSLNATSAA

Epitope

DALEHLEDHQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

337 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin Antibody (CL2968)

Western Blot: Laminin Antibody (CL2968) [NBP2-42384]

Western Blot: Laminin Antibody (CL2968) [NBP2-42384]

Western Blot: Laminin Antibody (CL2968) [NBP2-42384] - Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-221, Laminin-332, Laminin-411 and Laminin-521, using Laminin Antibody (CL2968) [NBP2-42384]. Observed molecular weight ~400 kDa. Theoretical Molecular Weight for NBP2-42384 is 337 kDa.
Western Blot: Laminin Antibody (CL2968) [NBP2-42384]

Western Blot: Laminin Antibody (CL2968) [NBP2-42384]

Western Blot: Laminin Antibody (CL2968) [NBP2-42384] - Analysis using Laminin Antibody (CL2968) [NBP2-42384]. Lane 1: Marker [kDa] 460, 268, 238, 171, 117, 71, 55, 41, 31 Lane 2: Human Testis tissue. Observed molecular weight ~400 kDa. Theoretical Molecular Weight for NBP2-42384 is 337 kDa.
Laminin Antibody (CL2968)

Immunohistochemistry-Paraffin: Laminin Antibody (CL2968) [NBP2-42384] -

Immunohistochemistry-Paraffin: Laminin Antibody (CL2968) [NBP2-42384] - Analysis in human testis and tonsil tissues using NBP2-42384 antibody. Corresponding LAMA1 RNA-seq data are presented for the same tissues.
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Applications for Laminin Antibody (CL2968)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y., . . . Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Alternate Names

LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha

Gene Symbol

LAMA1

UniProt

P25391

Additional Laminin Products

Product Documents for Laminin Antibody (CL2968)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin Antibody (CL2968)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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