Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Laminin Antibodies »

Product Specifications

Immunogen

Laminin Antibody was made to Laminin 111 isolated from mouse Engelbreth-Holm-Swarm (EHS) sarcoma cells. [UniProt# P19137]

Reactivity Notes

Rabbit, Fruit Bat, Chinese Hamster, and S. mansoni reactivity reported in scientific literature (PMID: 18214989, 31877588, 29251349, and 28114363 respectively). Human, Mouse, Rat, and Sheep reported in multiple pieces of scientific literature.

Localization

Secreted, Basement membrane, Extracellular matrix

Specificity

Laminin Antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).

Marker

Basement Membrane Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Laminin Antibody [Alexa Fluor® 647]

Frozen Mouse Tissue Stained for Laminin

SOX17 regulates adult muscle regeneration after injury in Pax7CreERT2/+;Sox17fl/fl mutant mice. Representative images of cryosections from regenerating adult TA muscles d28 after injury, showing immunofluorescence for PAX7+ (quiescent, arrows) cells. Scale bar, 25 Aum. Image collected and cropped by Citeab from the following publication (SOXF factors regulate murine satellite cell self-renewal and function through inhibition ofI2-catenin activity. Elife (2018)) licensed under a CC-BY license.

Flow Cytometry of HeLa Cells Stained with Conjugated Laminin Antibody

A surface stain was performed on HeLa cells with Laminin Antibody NB300-144AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - SOX17 regulates adult muscle regeneration after injury in Pax7CreERT2/+;Sox17fl/fl mutant mice.(A) Schematic outline of experimental procedure for tamoxifen (TMX) injection (i.p., intraperitoneal). CTX, cardiotoxin injection; d, days. (B) Representative images of cryosections from regenerating adult TA muscles d7 after injury, showing immunofluorescence for PAX7+ (quiescent, arrows) & PH3+PAX7+ (proliferating, arrowheads) cells. Scale bar, 25 μm. (C–D) Quantification of satellite cells as illustrated in (B). (E) Schematic outline of experimental procedure for TMX diet. CTX, cardiotoxin injection; d, days. (F) Representative images of cryosections from regenerating adult TA muscles d28 after injury, showing immunofluorescence for PAX7+ (quiescent, arrows) cells. Scale bar, 25 µm. (G) Quantification of satellite cells as illustrated in (F). (H–I) Quantification of cross-sectional area in µm2 (H) & myofiber number per mm2 (I). (J–K) Quantification of fat infiltration (Oil red O) (J) & fibrosis (Sirius red) (K) indicated as proportion of stained section (average of five sections per muscle). (L) Representative images of histological characterization of adult TA muscles 28 days after injury w/ Hematoxylin & eosin (cell infiltration; upper panel), Oil red O (fat infiltration; middle panel), & Sirius red (fibrosis; bottom panel) staining. Scale bars, 100 µm. CTRL, Sox17fl/fl; cKO, Pax7CreERT2/+;Sox17fl/fl. n ≥ 3 mice (each quantified at least in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed w/ Student’s unpaired t-test (C,D,G) & Mann-Whitney ranking test (H–K): n.s., not significant; *, p<0.05; **, p<0.01; ***, p<0.001, compared to CTRL. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/29882512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - SOX17 is necessary to maintain satellite cell quiescence in adult muscles.(A,F) Representative Soleus cryosection images showing immunofluorescence for satellite cells (PAX7+, arrows) in Pax3Cre/+;Sox17GFP/fl & Pax7CreERT2/+;Sox17fl/fl mice, with appropriate controls. Scale bars, 25 μm. Fibers are identified by LAMININ & nuclei are counterstained with DAPI. (B,G) Quantification of satellite cell number during postnatal growth (P14) & in adult. (C) Quantification of the ratio PAX7/MYOD+ satellite cells in P14 Soleus cryosections. (D) RT-qPCR analysis on adult TA muscles for Pax7 & SoxF genes in fresh FACS-isolated satellite cells from control & Sox17-knockout mice. (A–D) CTRL, Sox17GFP/fl; KO, Pax3Cre/+;Sox17GFP/fl. (E) Schematic outline of the experimental procedure for tamoxifen (TMX) injection (i.p., intraperitoneal) in Sox17fl/fl (CTRL) & Pax7CreERT2/+;Sox17fl/fl (cKO) mice. d, days. (E–G) CTRL, Sox17fl/fl; cKO, Pax7CreERT2/+;Sox17fl/fl. Quantification was performed in whole cross-sections. n ≥ 4 mice (each quantified in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed with Student’s unpaired t-test: *, p<0.05; **, p<0.01, compared to CTRL.Satellite cells characterization of control & Sox17-knockout mice.(A) Immunofluorescence of satellite cells (MCAD; M-cadherin) in adult Soleus cryosections from control & Sox17 mutant mice. Scale bar, 25 μm. (B) Quantification of satellite cell number illustrated in (A). CTRL, Sox17GFP/fl; KO, Pax3Cre/+;Sox17GFP/fl. n ≥ 4 mice (each quantified in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed with Student’s unpaired t-test: *, p<0.05, compared to CTRL. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29882512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - MuSC-specific Cdkn1c ablation hinders muscle regeneration.(A) Time-course of tamoxifen administration, intramuscular injury of TA muscle (CTX arrow), & muscle harvest (D7 arrow). (B–G) Cryosections of TA muscle were stained for histological & satellite cell population characterization 7 days after CTX injection. Analyzed animals at (B-G) were wild-type littermates (Wt; Pax7+; Cdkn1c+; B–G), Cre control (Pax7CreERT2; B’–G’), & Cdkn1c cKO (Pax7CreERT2; Cdkn1cFlox; B’’–G’’). (B) HE staining for histologic characterization of the muscles. (C) Oil Red O staining for evaluation of fat infiltration of the muscles. (D–E) embryonic myosin (eMYHC, red)/LAMININ (LAM, green) immunofluorescence to mark newly formed myofibers post-regeneration. (F–G) PAX7 (red)/LAMININ (LAM, green) immunofluorescence to mark PAX7+ satellite cells. Nuclei in (D-G) were counter-stained with DAPI (blue). Scale bars, 50 μm. (H) Quantification of (F-G). Data show mean +SD, n ≥ 5 animals. Asterisks indicate significance; **p≤0.01.In vivo MuSC-specific Cdkn1c ablation. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30284969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

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