Laminin beta 3 Antibody (2G10)
Novus Biologicals, part of Bio-Techne | Catalog # H00003914-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2G10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
LAMB3 (NP_000219, 1064 a.a. ~ 1171 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVEQIRDHINGRVLYYATC
Localization
Extracellular; found in the basement membranes
Specificity
LAMB3 - laminin, beta 3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Laminin beta 3 Antibody (2G10)
![Western Blot: Laminin beta 3 Antibody (2G10) [H00003914-M01]](https://resources.bio-techne.com/images/products/Laminin-beta-3-Antibody-2G10-Western-Blot-H00003914-M01-img0007.jpg "Western Blot: Laminin beta 3 Antibody (2G10) [H00003914-M01]")
Western Blot: Laminin beta 3 Antibody (2G10) [H00003914-M01]
Western Blot: Laminin beta 3 Antibody (2G10) [H00003914-M01] - LAMB3 monoclonal antibody (M01), clone 2G10 Analysis of LAMB3 expression in A-431.![Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (2G10) [H00003914-M01]](https://resources.bio-techne.com/images/products/Laminin-beta-3-Antibody-2G10-Immunocytochemistry-Immunofluorescence-H00003914-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (2G10) [H00003914-M01]")
Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (2G10) [H00003914-M01]
Immunocytochemistry/Immunofluorescence: Laminin beta 3 Antibody (2G10) [H00003914-M01] - Analysis of monoclonal antibody to LAMB3 on A-431 cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Laminin beta 3 Antibody (2G10) [H00003914-M01] - Detection limit for recombinant GST tagged LAMB3 is approximately 0.03ng/ml as a capture antibody.
Applications for Laminin beta 3 Antibody (2G10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Laminin beta 3
Alternate Names
BM600-125KDA, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa
Entrez Gene IDs
3914 (Human)
Gene Symbol
LAMB3
UniProt
Additional Laminin beta 3 Products
Product Documents for Laminin beta 3 Antibody (2G10)
Product Specific Notices for Laminin beta 3 Antibody (2G10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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