LAMP-1/CD107a Antibody (GT25212)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13609

Novus Biologicals, part of Bio-Techne
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NBP3-13609
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT25212

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LAMP-1/CD107a Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP-1/CD107a. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for LAMP-1/CD107a Antibody (GT25212)

Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609]

Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609]

Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609]

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609]

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - LAMP-1/CD107a antibody [GT25212] detects LAMP-1/CD107a protein at lysosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LAMP-1/CD107a stained by LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:2000. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609]

Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609]

Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - Wild-type (WT) and LAMP-1/CD107a knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
View 3 more images

Applications for LAMP-1/CD107a Antibody (GT25212)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Gene Symbol

LAMP1

Additional LAMP-1/CD107a Products

Product Documents for LAMP-1/CD107a Antibody (GT25212)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LAMP-1/CD107a Antibody (GT25212)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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