Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG1 Clone # RL13

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LAP1 Antibodies »

Product Specifications

Immunogen

Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

Localization

Immunofluorescence staining of LAP 1 in rat liver with this antibody results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.

Specificity

Detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat tissues. This does not cross-react with LAP 2.This detects 3 distinct proteins of molecular weights 75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with NB120-2737 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for LAP1 Antibody (RL13) [Janelia Fluor® 635]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: LAP1

In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others.LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only.In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.

Alternate Names

lamina-associated polypeptide 1B, torsin A interacting protein 1

Gene Symbol

TOR1AIP1

Additional LAP1 Products

Product Documents for LAP1 Antibody (RL13) [Janelia Fluor® 635]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LAP1 Antibody (RL13) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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