LASP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77338

Novus Biologicals, part of Bio-Techne
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NB100-77338
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LASP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PERRDSQDGSSYR corresponding to internal region according to NP_006139.1.

Epitope

PERRDSQDGSSYR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LASP1 Antibody

Western Blot: LASP1 Antibody [NB100-77338]

Western Blot: LASP1 Antibody [NB100-77338]

Western Blot: LASP1 Antibody [NB100-77338] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338]

Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338]

Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338]

Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338]

Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for LASP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03-0.1 ug/ml
Application Notes
Western blot: Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207(2):171-5; PMID: 9511759).
IHC: Paraffin embedded Human Colon and Small Intestine.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LASP1

This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Alternate Names

LASP-1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, Metastatic lymph node gene 50 protein, MLN 50, MLN50Lasp-1

Entrez Gene IDs

3927 (Human)

Gene Symbol

LASP1

UniProt

NP_006139.1 (Human)

Additional LASP1 Products

Product Documents for LASP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LASP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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