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LASS2 Antibody (HL1717) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25546

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25546

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1717

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for LASS2 Antibody (HL1717) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LASS2. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LASS2 Antibody (HL1717) - Azide and BSA Free

LASS2 Antibody (HL1717) - Azide and BSA Free

Western Blot: LASS2 Antibody (HL1717) - Azide and BSA Free [NBP3-25546] -

Western Blot: LASS2 Antibody (HL1717) - Azide and BSA Free [NBP3-25546] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (NBP3-25546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
LASS2 Antibody (HL1717) - Azide and BSA Free

Knockout Validated: LASS2 Antibody (HL1717) - Azide and BSA Free [NBP3-25546] -

Knockout Validated: LASS2 Antibody (HL1717) - Azide and BSA Free [NBP3-25546] - Wild-type (WT) and CERS2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (NBP3-25546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
LASS2 Antibody (HL1717) - Azide and BSA Free

Immunohistochemistry-Paraffin: LASS2 Antibody (HL1717) - Azide and BSA Free [NBP3-25546] -

Immunohistochemistry-Paraffin: LASS2 Antibody (HL1717) - Azide and BSA Free [NBP3-25546] - LASS2 antibody [HL1717] detects LASS2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LASS2 stained by LASS2 antibody [HL1717] (NBP3-25546) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for LASS2 Antibody (HL1717) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LASS2

LASS2 encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described.

Alternate Names

ceramide synthase 2, CerS2, LAG1 homolog, ceramide synthase 2, LAG1 longevity assurance 2, LAG1 longevity assurance homolog 2, LAG1 longevity assurance homolog 2 (S. cerevisiae), S. cerevisiae) homolog 2, SP260MGC987, TMSG1, Tumor metastasis-suppressor gene 1 protein

Gene Symbol

CERS2

Additional LASS2 Products

Product Documents for LASS2 Antibody (HL1717) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LASS2 Antibody (HL1717) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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