LASS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12946

Novus Biologicals, part of Bio-Techne
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NBP3-12946
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (93%), Rat (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LASS2 Antibodies »

Product Summary for LASS2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human LASS2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for LASS2 Antibody

Western Blot: LASS2 Antibody [NBP3-12946]

Western Blot: LASS2 Antibody [NBP3-12946]

Western Blot: LASS2 Antibody [NBP3-12946] - Wild-type (WT) and CERS2 knockout (KO) 293T WT cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody (NBP3-12946) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: LASS2 Antibody [NBP3-12946]

Immunocytochemistry/ Immunofluorescence: LASS2 Antibody [NBP3-12946]

Immunocytochemistry/Immunofluorescence: LASS2 Antibody [NBP3-12946] - LASS2 antibody detects LASS2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LASS2 protein stained by LASS2 antibody (NBP3-12946) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: LASS2 Antibody [NBP3-12946]

Western Blot: LASS2 Antibody [NBP3-12946]

Western Blot: LASS2 Antibody [NBP3-12946] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody (NBP3-12946) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Applications for LASS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LASS2

LASS2 encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described.

Alternate Names

ceramide synthase 2, CerS2, LAG1 homolog, ceramide synthase 2, LAG1 longevity assurance 2, LAG1 longevity assurance homolog 2, LAG1 longevity assurance homolog 2 (S. cerevisiae), S. cerevisiae) homolog 2, SP260MGC987, TMSG1, Tumor metastasis-suppressor gene 1 protein

Gene Symbol

CERS2

Additional LASS2 Products

Product Documents for LASS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LASS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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