Lck Antibody (LCK-01) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-336
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # LCK-01
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Localization
Cytoplasmic
Specificity
The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Lck Antibody (LCK-01) - BSA Free
Western Blot: Lck Antibody (LCK-01)BSA Free [NB500-336]
Western Blot: Lck Antibody (LCK-01) [NB500-336] - Fig. 1. Western Blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck Lane 2: HEK-293T cell line transfected with Lck Lane 3: HEK-293T cell line (non-transfected).Flow Cytometry: Lck Antibody (LCK-01) - BSA Free [NB500-336]
Flow Cytometry: Lck Antibody (LCK-01) [NB500-336] - Staining of Lck in human peripheral blood cells using anti-Lck (LCK-01) Alexa Fluor® 647.Applications for Lck Antibody (LCK-01) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1-5 ug/ml
Flow Cytometry
1-5 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:50
Western Blot
1:1000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Lck
Long Name
Lymphocyte Protein Tyrosine Kinase
Alternate Names
Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16
Gene Symbol
LCK
Additional Lck Products
Product Documents for Lck Antibody (LCK-01) - BSA Free
Product Specific Notices for Lck Antibody (LCK-01) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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