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LDB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85573

Novus Biologicals, part of Bio-Techne
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NBP1-85573
NBP1-85573-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LDB1 Antibody

Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-85573]

Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-85573]

Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-85573] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-85573] - Staining of human bone marrow shows strong nuclear positivity in a subset of hematopoietic cells.

Applications for LDB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LDB1

LDB1 binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neuron

Alternate Names

carboxy terminal LIM domain protein 2, Carboxyl-terminal LIM domain-binding protein 2, CLIM-2, CLIM2hLdb1, LDB-1, LIM domain binding 1, LIM domain-binding factor-1, LIM domain-binding protein 1, NLILIM domain-binding factor CLIM2, Nuclear LIM interactor

Gene Symbol

LDB1

Additional LDB1 Products

Product Documents for LDB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LDB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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