LDB2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-480
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified LDB2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2. (Uniprot: O55203)
Reactivity Notes
A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for LDB2 Antibody
![Western Blot: LDB2 Antibody [NB600-480]](https://resources.bio-techne.com/images/products/LDB2-Antibody-Western-Blot-NB600-480-img0006.jpg "Western Blot: LDB2 Antibody [NB600-480]")
Western Blot: LDB2 Antibody [NB600-480]
Western Blot: LDB2 Antibody [NB600-480] - Analysis of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.![Immunohistochemistry: LDB2 Antibody [NB600-480]](https://resources.bio-techne.com/images/products/LDB2-Antibody-Immunohistochemistry-NB600-480-img0005.jpg "Immunohistochemistry: LDB2 Antibody [NB600-480]")
Immunohistochemistry: LDB2 Antibody [NB600-480]
Immunohistochemistry: LDB2 Antibody [NB600-480] - Used at a 5 ug/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.Applications for LDB2 Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: LDB2
Alternate Names
Carboxyl-terminal LIM domain-binding protein 1, CLIM-1, CLIM1LDB1, LDB-2, LIM binding domain 2, LIM domain binding 2, LIM domain-binding factor CLIM1, LIM domain-binding factor-2, LIM domain-binding protein 2
Gene Symbol
LDB2
UniProt
Additional LDB2 Products
Product Documents for LDB2 Antibody
Product Specific Notices for LDB2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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