LDLR Antibody (032)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90523

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90523-100ul
NBP2-90523-50ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 032

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LDLR Antibodies »

Product Summary for LDLR Antibody (032)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LDLR (Met 1-Arg 790; Accession#: NP_034830.2).

Specificity

No cross-reactivity in ELISA with: Human LDLR / LDL Receptor

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LDLR Antibody (032)

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (032) [NBP2-90523]

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (032) [NBP2-90523]

Immunocytochemistry/Immunofluorescence: LDLR Antibody (032) [NBP2-90523] - Immunofluorescence staining of Mouse LDLR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse LDLR monoclonal antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG Secondary antibody(green), counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow Cytometry: LDLR Antibody (032) [NBP2-90523]

Flow Cytometry: LDLR Antibody (032) [NBP2-90523]

Flow Cytometry: LDLR Antibody (032) [NBP2-90523] - Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for LDLR Antibody (032)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LDLR

The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis. Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits. It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) were shown to be mediated by LDL receptors on cultured cells.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Gene Symbol

LDLR

Additional LDLR Products

Product Documents for LDLR Antibody (032)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LDLR Antibody (032)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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