LDLR Antibody (2B10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26172
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2B10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for LDLR Antibody (2B10)
Immunogen
A synthesized peptide derived from Human LDLR [UniProt P01130]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LDLR Antibody (2B10)
Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] -
Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] -
Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunocytochemistry/Immunofluorescence: LDLR Antibody (2B10) [NBP3-26172] -
Immunocytochemistry/Immunofluorescence: LDLR Antibody (2B10) [NBP3-26172] - Staining of Hela Cells with LDLR Antibody (2B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for LDLR Antibody (2B10)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: LDLR
Long Name
Low Density Lipoprotein Receptor
Alternate Names
LDL R
Gene Symbol
LDLR
Additional LDLR Products
Product Documents for LDLR Antibody (2B10)
Product Specific Notices for LDLR Antibody (2B10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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