LEDGF Antibody (3H1)

Novus Biologicals, part of Bio-Techne | Catalog # H00011168-M01

Novus Biologicals, part of Bio-Techne
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H00011168-M01
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LEDGF Antibodies »

Product Specifications

Immunogen

PSIP1 (AAH33817, 1 a.a. ~ 50 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHET

Reactivity Notes

Human. Other species not tested.

Specificity

PSIP1 - PC4 and SFRS1 interacting protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for LEDGF Antibody (3H1)

Western Blot: LEDGF Antibody (3H1) [H00011168-M01]

Western Blot: LEDGF Antibody (3H1) [H00011168-M01]

Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.
Immunohistochemistry-Paraffin: LEDGF Antibody (3H1) [H00011168-M01]

Immunohistochemistry-Paraffin: LEDGF Antibody (3H1) [H00011168-M01]

Immunohistochemistry-Paraffin: LEDGF Antibody (3H1) [H00011168-M01] - Analysis of monoclonal antibody to PSIP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Sandwich ELISA: LEDGF Antibody (3H1) [H00011168-M01] - Detection limit for recombinant GST tagged PSIP1 is approximately 1ng/ml as a capture antibody.

Applications for LEDGF Antibody (3H1)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LEDGF

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Long Name

Lens Epithelium-derived Growth Factor

Alternate Names

CLL-associated antigen KW-7, DFS 70, PSIP1, Transcriptional coactivator p75/p52

Entrez Gene IDs

11168 (Human)

Gene Symbol

PSIP1

OMIM

603620 (Human)

UniProt

AAH33817 (Human)

Additional LEDGF Products

Product Documents for LEDGF Antibody (3H1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LEDGF Antibody (3H1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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