LEF1 Antibody (LEF1/6764)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20278
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LEF1/6764
Concentration
0.2 mg/ml
Product Summary for LEF1 Antibody (LEF1/6764)
Immunogen
Recombinant fragment of human LEF1 protein (exact sequence is proprietary)
Localization
Nucleus.
Specificity
Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: Human tonsil and thymus.
Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for LEF1 Antibody (LEF1/6764)
LEF1 Antibody (LEF1/6764)
Analysis of Protein Array containing more than 19,000 full-length human proteins using LEF1-Monospecific Mouse Monoclonal Antibody (LEF1/6764).
LEF1 Antibody (LEF1/6764)
Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 antibody (LEF1/6764). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
LEF1 Antibody (LEF1/6764)
Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 antibody (LEF1/6764). Inset: PBS instead of primary antibody; secondary only negative control.Applications for LEF1 Antibody (LEF1/6764)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: LEF1
Long Name
Lymphoid Enhancer-binding Factor 1
Alternate Names
TCF10, TCF1ALPHA, TCF7L3
Gene Symbol
LEF1
UniProt
Additional LEF1 Products
Product Documents for LEF1 Antibody (LEF1/6764)
Product Specific Notices for LEF1 Antibody (LEF1/6764)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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