LEF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-41374
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]](https://resources.bio-techne.com/images/products/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0002.jpg "Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QHEQRKEQEPKRPH corresponding to internal region according to NP_057353.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for LEF1 Antibody
![Western Blot: LEF1 Antibody [NB100-41374]](https://resources.bio-techne.com/images/products/LEF1-Antibody-Western-Blot-NB100-41374-img0001.jpg "Western Blot: LEF1 Antibody [NB100-41374]")
Western Blot: LEF1 Antibody [NB100-41374]
Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]](https://resources.bio-techne.com/images/products/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0003.jpg "Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]")
Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]
Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).![Flow Cytometry: LEF1 Antibody [NB100-41374]](https://resources.bio-techne.com/images/products/LEF1-Antibody-Flow-Cytometry-NB100-41374-img0004.jpg "Flow Cytometry: LEF1 Antibody [NB100-41374]")
Flow Cytometry: LEF1 Antibody [NB100-41374]
Flow Cytometry: LEF1 Antibody [NB100-41374] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for LEF1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
1 - 3 ug/mL
Application Notes
Approx 50-55 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 44.2 kDa according to NP_057353.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LEF1
Long Name
Lymphoid Enhancer-binding Factor 1
Alternate Names
TCF10, TCF1ALPHA, TCF7L3
Gene Symbol
LEF1
UniProt
Additional LEF1 Products
Product Documents for LEF1 Antibody
Product Specific Notices for LEF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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