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Leptin/OB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59320

Novus Biologicals, part of Bio-Techne
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NBP1-59320

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Summary for Leptin/OB Antibody

Immunogen

Synthetic peptides corresponding to LEP(leptin) The peptide sequence was selected form the N terminal of LEP. Peptide sequence MHWGTLCGFLWLWPYLFYVQAVPIQKVQDDTKTLIKTIVTRINDISHTQS. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Leptin/OB Antibody

Western Blot: Leptin/OB Antibody [NBP1-59320]

Western Blot: Leptin/OB Antibody [NBP1-59320]

Western Blot: Leptin Antibody [NBP1-59320] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.
Immunohistochemistry-Paraffin: Leptin/OB Antibody [NBP1-59320]

Immunohistochemistry-Paraffin: Leptin/OB Antibody [NBP1-59320]

Immunohistochemistry-Paraffin: Leptin Antibody [NBP1-59320] - Human placenta tissue at an antibody concentration of 5 ug/ml.

Applications for Leptin/OB Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8ug/ml

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Leptin/OB

LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regula

Alternate Names

LEP, OB

Gene Symbol

LEP

OMIM

NP_000221 (Human)

UniProt

Additional Leptin/OB Products

Product Documents for Leptin/OB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Leptin/OB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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