Leptin R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-5593

Novus Biologicals, part of Bio-Techne
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NB120-5593
NB120-5593-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

10 mg/ml

View all Leptin R Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9421392). Rat reactivity reported in scientific literature (PMID: 12810558).

Localization

Type I membrane protein. Except for form E which could be soluble.

Specificity

Leptin Receptor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Leptin R Antibody

Western Blot: Leptin R Antibody [NB120-5593]

Western Blot: Leptin R Antibody [NB120-5593]

Western Blot: Leptin R Antibody [NB120-5593] - Analysis of both short and long forms of OB-R from mouse lung tissue extract.

Applications for Leptin R Antibody

Application
Recommended Usage

Western Blot

1:2000
Application Notes
WB: Detects bands of approx. 100 and 125 kDa, corresponding to the long and short forms (respectively) of leptin receptor OB-R from mouse brain extract. Two smaller bands of approx. 31 and 35 kDa are also detected, which cannot be competed out with immunogenic peptide.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

10 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Leptin R

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Long Name

Leptin Receptor

Alternate Names

B219, CD295, LEPR, LeptinR, OB R

Entrez Gene IDs

3953 (Human); 16847 (Mouse); 24536 (Rat)

Gene Symbol

LEPR

UniProt

P48357

Additional Leptin R Products

Product Documents for Leptin R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Leptin R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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