Leptin R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-5593

Novus Biologicals, part of Bio-Techne
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NB120-5593
NB120-5593-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

10 mg/ml

View all Leptin R Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9421392). Rat reactivity reported in scientific literature (PMID: 12810558).

Localization

Type I membrane protein. Except for form E which could be soluble.

Specificity

Leptin Receptor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Leptin R Antibody

Western Blot: Leptin R Antibody [NB120-5593]

Western Blot: Leptin R Antibody [NB120-5593]

Western Blot: Leptin R Antibody [NB120-5593] - Analysis of both short and long forms of OB-R from mouse lung tissue extract.

Applications for Leptin R Antibody

Application
Recommended Usage

Western Blot

1:2000
Application Notes
WB: Detects bands of approx. 100 and 125 kDa, corresponding to the long and short forms (respectively) of leptin receptor OB-R from mouse brain extract. Two smaller bands of approx. 31 and 35 kDa are also detected, which cannot be competed out with immunogenic peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

10 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Leptin R

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Long Name

Leptin Receptor

Alternate Names

B219, CD295, LEPR, LeptinR, OB R

Entrez Gene IDs

3953 (Human); 16847 (Mouse); 24536 (Rat)

Gene Symbol

LEPR

UniProt

P48357

Additional Leptin R Products

Product Documents for Leptin R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Leptin R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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