Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Leucyl-cystinyl Aminopeptidase/LNPEP Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Oxytocinase using the numbering given in entry NP_005566.2 (GeneID 4012).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Leucyl-cystinyl Aminopeptidase/LNPEP Antibody

Western Blot: Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [NBP2-32111]

Western Blot: Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [NBP2-32111]

Western Blot: Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [NBP2-32111] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-Oxytocinase antibody NBP2-32111 used for IP at 6 ug per reaction. Oxytocinase was also immunoprecipitated by rabbit anti-Oxytocinase antibody BL16227. For blotting immunoprecipitated Oxytocinase, NBP2-32111 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [NBP2-32111]

Western Blot: Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [NBP2-32111]

Western Blot: Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [NBP2-32111] - Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Oxytocinase antibody NBP2-32111 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Leucyl-cystinyl Aminopeptidase/LNPEP Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Leucyl-cystinyl Aminopeptidase/LNPEP

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Alternate Names

IRAP, Leucylcystinyl Aminopeptidase, LNPEP, Otase, Oxytocinase, P-LAP, Vasopressinase

Gene Symbol

LNPEP

UniProt

NP_005566.2 (Human)

Additional Leucyl-cystinyl Aminopeptidase/LNPEP Products

Product Documents for Leucyl-cystinyl Aminopeptidase/LNPEP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Leucyl-cystinyl Aminopeptidase/LNPEP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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