Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S283-7

Concentration

1 mg/ml

View all LGI1 Antibodies »

Product Summary for Lgi1 Antibody (S283-7)

Immunogen

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human and Rat.

Localization

Cell Junction , Golgi Apparatus , Endoplasmic Reticulum

Specificity

approx 50% identity with LGI2, LGI3 and LGI4.Detects approx 60kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Lgi1 Antibody (S283-7)

Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190]

Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190]

Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.
Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190]

Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190]

Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Lgi1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190]

Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190]

Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.
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Applications for Lgi1 Antibody (S283-7)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of LGI1 Antibody was sufficient for detection of LGI1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LGI1

The Lgi1 gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobicsegment representing a putative transmembrane domain with the amino terminus located outside the cell. It alsocontains leucine-rich repeats

Long Name

Leucine-Rich Glioma-Inactivated Protein 1

Alternate Names

ADLTE, ADPAEF, ADPEAF, BB130740, Epitempin, Epitempin-1, EPT, ETL1, IB1099

Gene Symbol

LGI1

UniProt

Q9JIA1.1 (Human)

Additional LGI1 Products

Product Documents for Lgi1 Antibody (S283-7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lgi1 Antibody (S283-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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