Lgl1 Antibody (5G2)
Novus Biologicals, part of Bio-Techne | Catalog # H00003996-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5G2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
LLGL1 (NP_004131, 911 a.a. ~ 1010 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GIASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDINWPRDATQASYRIRESPKLSQANGTPSILLAPQSLDGSPDPAHSMGPDTPEPPEAALSP
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 21785947).
Specificity
LLGL1 - lethal giant larvae homolog 1 (Drosophila)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Lgl1 Antibody (5G2)
![Western Blot: Lgl1 Antibody (5G2) [H00003996-M01]](https://resources.bio-techne.com/images/products/Lgl1-Antibody-5G2-Western-Blot-H00003996-M01-img0001.jpg "Western Blot: Lgl1 Antibody (5G2) [H00003996-M01]")
Western Blot: Lgl1 Antibody (5G2) [H00003996-M01]
Western Blot: Lgl1 Antibody (5G2) [H00003996-M01] - Western Blot analysis of LLGL1 expression in Hela NE ( Cat # L013V3 ).![ELISA: Lgl1 Antibody (5G2) [H00003996-M01]](https://resources.bio-techne.com/images/products/Lgl1-Antibody-5G2-ELISA-H00003996-M01-img0004.jpg "ELISA: Lgl1 Antibody (5G2) [H00003996-M01]")
ELISA: Lgl1 Antibody (5G2) [H00003996-M01]
ELISA: Lgl1 Antibody (5G2) [H00003996-M01] - Detection limit for recombinant GST tagged LLGL1 is approximately 0.03ng/ml as a capture antibody.Applications for Lgl1 Antibody (5G2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using H00003996-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Lgl1
Alternate Names
DLG4lethal giant larvae (Drosophila) homolog 1, HUGL, HUGL1, Hugl-1, Human homolog to the D-lgl gene protein, lethal giant larvae homolog 1 (Drosophila), lethal(2) giant larvae protein homolog 1, LLGL
Entrez Gene IDs
3996 (Human)
Gene Symbol
LLGL1
OMIM
600966 (Human)
UniProt
Additional Lgl1 Products
Product Documents for Lgl1 Antibody (5G2)
Product Specific Notices for Lgl1 Antibody (5G2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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