Lgl1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84077

Novus Biologicals, part of Bio-Techne
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NBP1-84077
NBP1-84077-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lgl1 Antibodies »

Product Summary for Lgl1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FERFSLSARNITEPLCSLDINWPRDATQASYRIRESPKLSQANGTPSILLAPQSLDGSPDPAHSMGPDTPEPPEAALSPMSIDSATSADTTLDTTGDVTVEDVKDFLGSSEESEKNLRNLAEDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lgl1 Antibody

Western Blot: Lgl1 Antibody [NBP1-84077]

Western Blot: Lgl1 Antibody [NBP1-84077]

Western Blot: Lgl1 Antibody [NBP1-84077] - Analysis in human cell line U-251 MG.
Immunohistochemistry-Paraffin: Lgl1 Antibody [NBP1-84077]

Immunohistochemistry-Paraffin: Lgl1 Antibody [NBP1-84077]

Immunohistochemistry-Paraffin: Lgl1 Antibody [NBP1-84077] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Lgl1 Antibody [NBP1-84077]

Immunohistochemistry-Paraffin: Lgl1 Antibody [NBP1-84077]

Immunohistochemistry-Paraffin: Lgl1 Antibody [NBP1-84077] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
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Applications for Lgl1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lgl1

Lgl1 encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Alternate Names

DLG4lethal giant larvae (Drosophila) homolog 1, HUGL, HUGL1, Hugl-1, Human homolog to the D-lgl gene protein, lethal giant larvae homolog 1 (Drosophila), lethal(2) giant larvae protein homolog 1, LLGL

Entrez Gene IDs

3996 (Human)

Gene Symbol

LLGL1

UniProt

Q15334 (Human)

Additional Lgl1 Products

Product Documents for Lgl1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lgl1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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