Lgr5/GPR49 Antibody (707042) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB8078CL1
Published as Clone RD42
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Agonist Activity, CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # 707042
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
Met1-Ile560
Accession # O75473
Met1-Ile560
Accession # O75473
Specificity
Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Lgr5/GPR49 Antibody (707042) [CoraFluor™ 1]
Application
Recommended Usage
Agonist Activity
Optimal dilutions of this antibody should be experimentally determined.
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Lgr5/GPR49
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 5
Alternate Names
FEX, GPR49, GPR67, HG38
Gene Symbol
LGR5
Additional Lgr5/GPR49 Products
Product Documents for Lgr5/GPR49 Antibody (707042) [CoraFluor™ 1]
Product Specific Notices for Lgr5/GPR49 Antibody (707042) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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