Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Lgr5/GPR49 Antibodies »

Product Specifications

Immunogen

Synthetic peptide [KLH conjugated] made to the 2nd cytoplasmic loop of human GPR49/LGR5. [UniProt# O75473]

Reactivity Notes

Immunogen sequence has 81% identity with mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lgr5/GPR49 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1235] - GPR49/LGR5 Antibody [NLS1235] - Staining of a FFPE section of human cortex.
Immunohistochemistry: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry: Lgr5/GPR49 Antibody [NLS1235] - Stem cells, neutrophils and eosinophils in human bone marrow (1ug/ml).

Applications for Lgr5/GPR49 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

5-8 ug/ml

Immunohistochemistry-Paraffin

5-8 ug/ml
Application Notes
This GPR49/LGR5 antibody is useful for Immunohistochemistry on paraffin sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lgr5/GPR49

GPR49/LGR5 (G protein-coupled receptor 49 / leucine-rich repeat-containing G protein-coupled receptor 5), also called FEX, GPR67, GRP49, HG38 or MGC117008), is structurally related to members of glycoprotein hormone receptor family including FSH-R, TSH-R and LH-R. LGRs (leucine-rich repeat-containing G-protein coupled receptor) are divided into three major subgroups: glycoprotein hormone receptors; the subgroup of GPR48, GPR49, and LGR6; and receptors of relaxin family ligands (LGR7 and LGR8), of which GPR49 is emerging as a significant player in stem cells biology as it is highly expressed on stem cells in small intestine, colon and hair follicle. Deficiency of GPR49 in mice has been shown to cause neonatal lethality characterized by ankyloglossia, gastrointestinal distension and induction of premature differentiation of Paneth cells. LGR5 is a downstream target gene of Wnt signaling pathway (critical for morphogenesis and tumorigenesis) and it has also been reported as a receptor for R-spondins which are potent mediators of Wnt/beta-catenin as well as Wnt/PCP signaling. Accordingly, when compared to normal tissues, LGR5 is found upregulated in certain cancers e.g liver cancer with beta-catenin mutations, basal cell carcinoma, colon and ovarian carcinomas.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human)

Gene Symbol

LGR5

UniProt

O75473 (Human)

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lgr5/GPR49 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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