LHR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76804

Novus Biologicals, part of Bio-Techne
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NBP2-76804
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LHR Antibodies »

Product Summary for LHR Antibody

Immunogen

Peptide with sequence CQGTALLDKTRYTE corresponding to C-Terminus according to NP_000224.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LHR Antibody

Flow Cytometry: LHR Antibody [NBP2-76804]

Flow Cytometry: LHR Antibody [NBP2-76804]

Flow Cytometry: LHR Antibody [NBP2-76804] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: LHR Antibody [NBP2-76804]

Immunofluorescence: LHR Antibody [NBP2-76804]

Immunofluorescence: LHR Antibody [NBP2-76804] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Applications for LHR Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection Limit 1:32000

Western Blot

0.3 - 0.1 ug/ml
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in lysates of human brain, placenta and prostate and of cell lines Daudi, HeLa and K562 after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.6 kDa band according to NP_000224.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LHR

LHCGR encodes the receptor for both luteinizing hormone and choriogonadotropin. Mutations in this gonadotropin receptor result in disorders of male secondary sexual character development, such as familial male precocious puberty and male pseudohermaphtoditism.

Long Name

Luteinizing Hormone Receptor/Choriogonadotropin Receptor

Alternate Names

CGR, HHG, LCGR, LGR2, LHCGR, ULG5

Entrez Gene IDs

3973 (Human)

Gene Symbol

LHCGR

UniProt

P22888 (Human), NP_000224.2 (Human)

Additional LHR Products

Product Documents for LHR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LHR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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