LIM kinase 2 [p Thr505] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82023

Novus Biologicals, part of Bio-Techne
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NB100-82023
NB100-82023-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all LIM kinase 2 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human LIM Kinase 2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).

Modification

p Thr505

Specificity

Detects endogenous levels of LIM kinase 2 only when phosphorylated at threonine 505.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIM kinase 2 [p Thr505] Antibody

Western Blot: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Analysis of extracts from Hela cells untreated or treated with PMA using LIM kinase 2 [p Thr505] Antibody.
Immunocytochemistry/Immunofluorescence: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Staining of methanol-fixed Hela cells using LIM kinase 2 [p Thr505] Antibody.

Immunohistochemistry-Paraffin: LIM kinase 2 [p Thr505] Antibody [NB100-82023] -

Immunohistochemistry-Paraffin: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-82023 (left) or the same antibody preincubated with blocking peptide(right).

Applications for LIM kinase 2 [p Thr505] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIM kinase 2

LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.

Long Name

LIM domain kinase 2

Alternate Names

LIMK-2, LIMK2

Entrez Gene IDs

3985 (Human)

Gene Symbol

LIMK2

UniProt

P53671 (Human)

Additional LIM kinase 2 Products

Product Documents for LIM kinase 2 [p Thr505] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LIM kinase 2 [p Thr505] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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