LIM1 Antibody (OTI2D5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71134

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LIM1 Antibodies »

Product Summary for LIM1 Antibody (OTI2D5) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIM1 Antibody (OTI2D5) - Azide and BSA Free

Western Blot: LIM1 Antibody (OTI2D5)Azide and BSA Free [NBP2-71134]

Western Blot: LIM1 Antibody (OTI2D5)Azide and BSA Free [NBP2-71134]

Western Blot: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.
Immunocytochemistry/ Immunofluorescence: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134]

Immunocytochemistry/ Immunofluorescence: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134]

Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.
Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134]

Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134]

Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.
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Applications for LIM1 Antibody (OTI2D5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIM1

The LIM1 gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development o

Long Name

LIM Homeobox 1

Alternate Names

LHX1

Gene Symbol

LHX1

Additional LIM1 Products

Product Documents for LIM1 Antibody (OTI2D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIM1 Antibody (OTI2D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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