LIM1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-12933
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Amphibian, Fish
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to C-terminal portion of frog Lim-1.
Reactivity Notes
Frog. Also reacts with Lim-1 from whole-mount fish embryo.
Localization
Nuclear
Specificity
Does not appear to crossreact with LIM5 (the closest homologue of LIM1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
For NB110-12933 specific protocl, please see the PDF under the Product PDFs.
Scientific Data Images for LIM1 Antibody - BSA Free
![Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933]](https://resources.bio-techne.com/images/products/LIM1-Antibody---BSA-Free-Immunohistochemistry-NB110-12933-img0001.jpg "Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933]")
Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933]
Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA FreeApplications for LIM1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200-1:1000
Immunohistochemistry Whole-Mount
1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Immunoprecipitation
1:200
Western Blot
1:3000-1:6000
Application Notes
Immunohistochemistry (Paraffin): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative MEMFA, Immunocytochemistry: 1:500 on P19 cell line. Optimal dilutions to be determined by the researcher.
Do not use tissues fixed overnight in PFA, the antibody will not work.
Do not use tissues fixed overnight in PFA, the antibody will not work.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02M PBS, pH 7.6
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LIM1
Long Name
LIM Homeobox 1
Alternate Names
LHX1
Gene Symbol
LHX1
UniProt
Additional LIM1 Products
Product Documents for LIM1 Antibody - BSA Free
Product Specific Notices for LIM1 Antibody - BSA Free
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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