LIMS2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52185
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RWSNMSDALA corresponding to internal region according to NP_001129509.2, NP_001154875.1.
Epitope
C-RWSNMSDALA
Specificity
This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for LIMS2 Antibody
![Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP1-52185]](https://resources.bio-techne.com/images/products/LIMS2-Antibody-Immunohistochemistry-Paraffin-NBP1-52185-img0001.jpg "Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP1-52185]")
Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP1-52185]
Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP1-52185] - 3.75ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for LIMS2 Antibody
Application
Recommended Usage
Immunohistochemistry
0.5 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
detection limit 1:8000
Application Notes
Western blot: Preliminary experiments gave an approx 80kDa band in Human Heart and Mouse Heart lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_001129509.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Spleen.
IHC: Paraffin embedded Human Spleen.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LIMS2
Alternate Names
FLJ10044, ILK-binding protein, LIM and senescent cell antigen-like domains 2, LIM and senescent cell antigen-like-containing domain protein 2, LIM-like protein 2, Particularly interesting new Cys-His protein 2, PINCH2, PINCH-2
Entrez Gene IDs
55679 (Human)
Gene Symbol
LIMS2
UniProt
Additional LIMS2 Products
Product Documents for LIMS2 Antibody
Product Specific Notices for LIMS2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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