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LIMS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31713

Novus Biologicals, part of Bio-Techne
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NBP2-31713
NBP2-31713-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KRLKKLSELTSRKAQPKATDLNSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIMS2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMS2 Antibody [NBP2-31713]

Immunocytochemistry/ Immunofluorescence: LIMS2 Antibody [NBP2-31713]

Immunocytochemistry/Immunofluorescence: LIMS2 Antibody [NBP2-31713] - Staining of human cell line BJ shows localization to focal adhesion sites.
Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713]

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713]

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Staining of human skin shows low positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713]

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713]

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Analysis in human prostate and pancreas tissues. Corresponding LIMS2 RNA-seq data are presented for the same tissues.

Applications for LIMS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIMS2

LIMS2 is a focal adhesion protein that associates with integrin-linked kinase (ILK; MIM 602366), a multidomain protein that mediates multiple protein-protein interactions at adhesion sites between cells and the extracellular matrix (ECM).(supplied by OMIM)

Alternate Names

FLJ10044, ILK-binding protein, LIM and senescent cell antigen-like domains 2, LIM and senescent cell antigen-like-containing domain protein 2, LIM-like protein 2, Particularly interesting new Cys-His protein 2, PINCH2, PINCH-2

Gene Symbol

LIMS2

UniProt

Additional LIMS2 Products

Product Documents for LIMS2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LIMS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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