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LIN-28B Antibody (SR1509)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21675

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
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NBP3-21675-100ul
NBP3-21675-50ul

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1509

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human LIN-28B (Uniprot #: Q6ZN17)

Specificity

Detects endogenous levels of total LIN-28B

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIN-28B Antibody (SR1509)

Immunocytochemistry/Immunofluorescence: LIN-28B Antibody (SR1509) [NBP3-21675] -

Immunocytochemistry/Immunofluorescence: LIN-28B Antibody (SR1509) [NBP3-21675] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21675.

Western Blot: LIN-28B Antibody (SR1509) [NBP3-21675] -

Western Blot: LIN-28B Antibody (SR1509) [NBP3-21675] - Western blot analysis using NBP3-21675 in K562 cell lysate.

Applications for LIN-28B Antibody (SR1509)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIN-28B

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7),a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminaluridylation of pre-let-7. Urid

Long Name

LIN-28 Homolog B

Alternate Names

CSDD2, Lin-28.2, LIN28B

Gene Symbol

LIN28B

Additional LIN-28B Products

Product Documents for LIN-28B Antibody (SR1509)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LIN-28B Antibody (SR1509)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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