LIN-28B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32352

Novus Biologicals, part of Bio-Techne
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NBP2-32352
NBP2-32352-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LIN-28B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QEARAEISERSGRSPQEASSTKSSIAPEEQSKKGPSVQKRKKT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIN-28B Antibody

Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352]

Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352]

Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352]

Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352]

Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP2-32352 (A) shows similar protein distribution across tissues to independent antibody NBP1-85438 (B).
Western Blot: LIN-28B Antibody [NBP2-32352]

Western Blot: LIN-28B Antibody [NBP2-32352]

Western Blot: LIN-28B Antibody [NBP2-32352] - Analysis in human cell line HEK 293 and human cell line MCF-7.
View 5 more images

Applications for LIN-28B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIN-28B

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7),a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminaluridylation of pre-let-7. Urid

Long Name

LIN-28 Homolog B

Alternate Names

CSDD2, Lin-28.2, LIN28B

Gene Symbol

LIN28B

Additional LIN-28B Products

Product Documents for LIN-28B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIN-28B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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