Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIN7 Antibodies »

Product Summary for LIN7 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human LIN7.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LIN7 Antibody

Western Blot: LIN7 Antibody [NBP2-99481]

Western Blot: LIN7 Antibody [NBP2-99481]

Western Blot: LIN7 Antibody [NBP2-99481] - Lane A: Mouse brain tissue lysateLane B: Rat brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa
Immunocytochemistry/ Immunofluorescence: LIN7 Antibody [NBP2-99481]

Immunocytochemistry/ Immunofluorescence: LIN7 Antibody [NBP2-99481]

Immunocytochemistry/Immunofluorescence: LIN7 Antibody [NBP2-99481] - Immunofluorescence staining of LIN7 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human LIN7 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.
Immunoprecipitation: LIN7 Antibody [NBP2-99481]

Immunoprecipitation: LIN7 Antibody [NBP2-99481]

Immunoprecipitation: LIN7 Antibody [NBP2-99481] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATP6V1C1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :45 kDa

Applications for LIN7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIN7

LIN7 plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the corr

Alternate Names

hLin-7, LIN7, lin-7 homolog A (C. elegans), Lin-7A, MALS1, MALS-1MGC148143, Mammalian lin-seven protein 1, Tax interaction protein 33, TIP-33mammalian LIN-7 1, veli-1, VELI1protein lin-7 homolog A, vertebrate LIN7 homolog 1, Vertebrate lin-7 homolog 1

Gene Symbol

LIN7A

Additional LIN7 Products

Product Documents for LIN7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIN7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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