LIN7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99481
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human LIN7.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for LIN7 Antibody
Western Blot: LIN7 Antibody [NBP2-99481]
Western Blot: LIN7 Antibody [NBP2-99481] - Lane A: Mouse brain tissue lysateLane B: Rat brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDaImmunocytochemistry/ Immunofluorescence: LIN7 Antibody [NBP2-99481]
Immunocytochemistry/Immunofluorescence: LIN7 Antibody [NBP2-99481] - Immunofluorescence staining of LIN7 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human LIN7 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.Immunoprecipitation: LIN7 Antibody [NBP2-99481]
Immunoprecipitation: LIN7 Antibody [NBP2-99481] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATP6V1C1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :45 kDaApplications for LIN7 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Immunoprecipitation
5-10 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LIN7
Alternate Names
hLin-7, LIN7, lin-7 homolog A (C. elegans), Lin-7A, MALS1, MALS-1MGC148143, Mammalian lin-seven protein 1, Tax interaction protein 33, TIP-33mammalian LIN-7 1, veli-1, VELI1protein lin-7 homolog A, vertebrate LIN7 homolog 1, Vertebrate lin-7 homolog 1
Gene Symbol
LIN7A
Additional LIN7 Products
Product Documents for LIN7 Antibody
Product Specific Notices for LIN7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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