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Lipocalin-2/NGAL Antibody (14) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30002

Novus Biologicals, part of Bio-Techne
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NBP1-30002-0.025mg

Key Product Details

Species Reactivity

Human, Porcine, Canine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 14

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human NGAL

Epitope

Differs from those of NBP2-41364, NBP2-41365, NBP2-41368 antibodies.

Specificity

Binds human NGAL (neutrophil gelatinase-associated lipocalin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Lipocalin-2/NGAL Antibody (14) - BSA Free

ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP1-30002]

ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP1-30002]

ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP1-30002] - The calibration curve of a sandwich assay for Human Dimer NGAL using NBP1-30002 as the capture antibody and NBP1-30002B as the biotinylated detection antibody.

Applications for Lipocalin-2/NGAL Antibody (14) - BSA Free

Application
Recommended Usage

ELISA

1:4000

Western Blot

1:1000
Application Notes
ELISA: NBP1-30002 binds human NGAL in solution and coated onto a solid phase. NBP1-30002 (as capture antibody) forms sandwich pairs with NBP2-23529, NBP2-23531, NBP1-30000,NBP1-30003 and NBP1-30004 (as biotinylated detection antibodies) for measuring human NGAL. With NBP1-30003B detection antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis. With NBP1-30002B detection antibody, the assay is specific for NGAL homodimer and can be used at sample dilutions giving low levels of total NGAL. WB: NBP1-30002 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lipocalin-2/NGAL

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances. Pig NGAL may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Gene Symbol

LCN2

Additional Lipocalin-2/NGAL Products

Product Documents for Lipocalin-2/NGAL Antibody (14) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lipocalin-2/NGAL Antibody (14) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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