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Lipolysis Stimulated Lipoprotein Receptor Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89631

Novus Biologicals, part of Bio-Techne
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NBP1-89631
NBP1-89631-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Porcine, Rhesus Macaque

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRSRAYMPPRSRSRDDLYDQDDS

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25753034). Primate (Rhesus macaques) reported in scientific literature (PMID: 26067369). Porcine reactivity reported in scientific literature (PMID: 24048008)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lipolysis Stimulated Lipoprotein Receptor Antibody

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]

Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining in human stomach and kidney tissues . Corresponding LSR RNA-seq data are presented for the same tissues.
Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]

Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]

Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]

Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]

Immunocytochemistry/Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.

Applications for Lipolysis Stimulated Lipoprotein Receptor Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Knockdown Validated

Reported in scientific publication (PMID: 32423802).

Simple Western

1:20

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue, internal validation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LSR

Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells

Long Name

Lipolysis Stimulated Lipoprotein Receptor

Alternate Names

ILDR3, LISCH, LISCH7

Gene Symbol

LSR

Additional LSR Products

Product Documents for Lipolysis Stimulated Lipoprotein Receptor Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lipolysis Stimulated Lipoprotein Receptor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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