Lipoprotein Lipase/LPL Antibody (OTI3A10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01395

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A10

Concentration

1 mg/ml

View all Lipoprotein Lipase/LPL Antibodies »

Product Summary for Lipoprotein Lipase/LPL Antibody (OTI3A10)

Immunogen

Human recombinant protein fragment corresponding to amino acids 28-475 of human LPL(NP_000228) produced in HEK293T.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lipoprotein Lipase/LPL Antibody (OTI3A10)

Western Blot: Lipoprotein Lipase/LPL Antibody (OTI3A10) [NBP2-01395]

Western Blot: Lipoprotein Lipase/LPL Antibody (OTI3A10) [NBP2-01395]

Western Blot: Lipoprotein Lipase/LPL Antibody (3A10) [NBP2-01395] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoprotein lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lipoprotein lipase.
Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody (OTI3A10) [NBP2-01395]

Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody (OTI3A10) [NBP2-01395]

Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody (3A10) [NBP2-01395] - Human endometrium tissue using anti-Lipoprotein lipase mouse monoclonal antibody.
Flow Cytometry: Lipoprotein Lipase/LPL Antibody (OTI3A10) [NBP2-01395]

Flow Cytometry: Lipoprotein Lipase/LPL Antibody (OTI3A10) [NBP2-01395]

Flow Cytometry: Lipoprotein Lipase/LPL Antibody (3A10) [NBP2-01395] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Lipoprotein lipase antibody, and then analyzed by flow cytometry.
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Applications for Lipoprotein Lipase/LPL Antibody (OTI3A10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lipoprotein Lipase/LPL

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq].

Alternate Names

LIPD, LPL

Entrez Gene IDs

4023 (Human)

Gene Symbol

LPL

Additional Lipoprotein Lipase/LPL Products

Product Documents for Lipoprotein Lipase/LPL Antibody (OTI3A10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lipoprotein Lipase/LPL Antibody (OTI3A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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