Lipoprotein Lipase/LPL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32215

Novus Biologicals, part of Bio-Techne
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NBP1-32215
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken, Sheep

Predicted:

Bovine (91%), Feline (94%), Porcine (93%), Rabbit (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lipoprotein Lipase/LPL Antibodies »

Product Summary for Lipoprotein Lipase/LPL Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lipoprotein Lipase/LPL. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Guinea Pig (86%).

Localization

Cell membrane, Lipid-anchor, GPI-anchor, Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lipoprotein Lipase/LPL Antibody

Western Blot: Lipoprotein Lipase/LPL Antibody [NBP1-32215]

Western Blot: Lipoprotein Lipase/LPL Antibody [NBP1-32215]

Western Blot: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - NIH-3T3 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: Lipoprotein Lipase/LPL Antibody [NBP1-32215]

Immunocytochemistry/ Immunofluorescence: Lipoprotein Lipase/LPL Antibody [NBP1-32215]

Immunocytochemistry/Immunofluorescence: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody [NBP1-32215]

Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody [NBP1-32215]

Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - Mouse white adipocyte. LPL stained by LPL antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for Lipoprotein Lipase/LPL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lipoprotein Lipase/LPL

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.

Alternate Names

LIPD, LPL

Gene Symbol

LPL

Additional Lipoprotein Lipase/LPL Products

Product Documents for Lipoprotein Lipase/LPL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lipoprotein Lipase/LPL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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