LIS1 Antibody (JE37-43)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32540
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE37-43
Concentration
1 mg/ml
Product Summary for LIS1 Antibody (JE37-43)
Immunogen
Synthetic peptide. (Uniprot: P43034)
Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, Nucleus membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LIS1 Antibody (JE37-43)
Western Blot: LIS1 Antibody (JE37-43) [NBP3-32540]
Western Blot: LIS1 Antibody (JE37-43) [NBP3-32540] - Western blot analysis of LIS1 on different lysates with Rabbit anti-LIS1 antibody (NBP3-32540) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutes; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32540) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for LIS1 Antibody (JE37-43)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LIS1
Alternate Names
LIS-1, LIS1PAF-AH alpha, LIS2, lissencephaly 1 protein, Lissencephaly-1 protein, MDCR, MDS, Miller-Dieker syndrome chromosome region, PAF acetylhydrolase 45 kDa subunit, PAFAH, PAF-AH 45 kDa subunit, PAFAH alpha, PAFAHA, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa), platelet-activating factor acetylhydrolase IB subunit alpha, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
Gene Symbol
PAFAH1B1
Additional LIS1 Products
Product Documents for LIS1 Antibody (JE37-43)
Product Specific Notices for LIS1 Antibody (JE37-43)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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