LIS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1040

Novus Biologicals, part of Bio-Techne
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NB100-1040
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LIS1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence TGSVDQTVKVWECR corresponding to C-Terminus according to NP_000421.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LIS1 Antibody

Western Blot: LIS1 Antibody [NB100-1040]

Western Blot: LIS1 Antibody [NB100-1040]

Western Blot: LIS1 Antibody [NB100-1040] - Staining of Rat Ovary lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: LIS1 Antibody [NB100-1040]

Immunohistochemistry-Paraffin: LIS1 Antibody [NB100-1040]

Immunohistochemistry-Paraffin: LIS1 Antibody [NB100-1040] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: LIS1 Antibody [NB100-1040]

Immunohistochemistry-Paraffin: LIS1 Antibody [NB100-1040]

Immunohistochemistry-Paraffin: LIS1 Antibody [NB100-1040] - Staining of paraffin embedded Human Pancreas. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for LIS1 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 40 kDa band observed in rat ovary lysates (calculated MW of 46.6 kDa band according to rat NP_113951.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIS1

Lissencephaly (LIS), literally meaning smooth brain, has multiple causes. Agyria, i.e., brain without convolutions or gyri, was considered a rare malformation until recent progress in neuroradiology. With this technical advantage, a number of lissencephaly syndromes have been distinguished. Classic lissencephaly (type I) is a brain malformation caused by abnormal neuronal migration at 9 to 13 weeks' gestation, resulting in a spectrum of agyria, mixed agyria / pachygyria, and pachygyria. It is characterized by an abnormally thick and poorly organized cortex with 4 primitive layers, diffuse neuronal heterotopia, enlarged and dysmorphic ventricles, and often hypoplasia of the corpus callosum. Lissencephaly is found in association with facial abnormalities in Miller-Dieker syndrome and without other major anomalies in X-linked lissencephaly and isolated lissencephaly sequence (ILS). Subcortical band heterotopia (SBH) are bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface and comprise the less severe end of the lissencephaly spectrum of malformations.

Alternate Names

LIS-1, LIS1PAF-AH alpha, LIS2, lissencephaly 1 protein, Lissencephaly-1 protein, MDCR, MDS, Miller-Dieker syndrome chromosome region, PAF acetylhydrolase 45 kDa subunit, PAFAH, PAF-AH 45 kDa subunit, PAFAH alpha, PAFAHA, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa), platelet-activating factor acetylhydrolase IB subunit alpha, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

Entrez Gene IDs

5048 (Human); 18472 (Mouse)

Gene Symbol

PAFAH1B1

UniProt

NP_000421 (Human)

Additional LIS1 Products

Product Documents for LIS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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