LKB1/STK11 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14835

Novus Biologicals, part of Bio-Techne
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NBP2-14835
NBP2-14835SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.2 mg/ml

View all LKB1/STK11 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a internal portion of the human LKB1 protein (between residues 250-350) [UniProt Q15831]

Reactivity Notes

Immunogen has 100% homology to rat.

Localization

The inactive form localizes to the nucleus; upon activation LKB1 translocates to the cytoplasm and mitochondria.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LKB1/STK11 Antibody - BSA Free

Western Blot: LKB1/STK11 AntibodyBSA Free [NBP2-14835]

Western Blot: LKB1/STK11 AntibodyBSA Free [NBP2-14835]

Western Blot: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in human testes lysate.
Immunocytochemistry/ Immunofluorescence: LKB1/STK11 Antibody - BSA Free [NBP2-14835]

Immunocytochemistry/ Immunofluorescence: LKB1/STK11 Antibody - BSA Free [NBP2-14835]

Immunocytochemistry/Immunofluorescence: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: LKB1/STK11 Antibody - BSA Free [NBP2-14835]

Immunohistochemistry-Paraffin: LKB1/STK11 Antibody - BSA Free [NBP2-14835]

Immunohistochemistry-Paraffin: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.

Applications for LKB1/STK11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 ug/ml
Application Notes
This LKB1 antibody is useful for Immunocytochemistry/Immunofluorescence, IHC-paraffin embedded sections and Western blot. In Western blot a band is detected ~48 kDa in human testes. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse stomach. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, nuclear staining was observed in HepG2 cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LKB1/STK11

Liver kinase B1 (LKB1), also known as serine/threonine-protein kinase STK11, is a member of the serine/threonine kinase family. LKB1 is a tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. More specifically, LKB1 controls the activity of AMP-activated protein kinase (AMPK) family members, regulates cell polarity by remodeling the actin cytoskeleton, regulates the inhibition of cell growth signaling pathways in response to low energy levels, regulates autophagy in response to nutrient deprivation, and is involved in DNA damage response by differentiating B-cells in the germinal center. LKB1 phosphorylation in cells occurs at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363. LKB1 is ubiquitously expressed in tissues with the strongest expression in testis and fetal liver. Defects in the LKB1 gene have been known to cause Peutz-Jeghers syndrome (PJS), testicular germ cell tumors (TGCT), non-small cell lung cancer (NSCLC), and other sporadic cancers. While not thoroughly characterized, alternate transcriptional splice variants of the LKB1 gene have been observed.

Long Name

Serine/threonine Protein Kinase LKB1

Alternate Names

PJS, STK11

Entrez Gene IDs

6794 (Human); 20869 (Mouse)

Gene Symbol

STK11

UniProt

Q15831, Q9WTK7 (Mouse)

Additional LKB1/STK11 Products

Product Documents for LKB1/STK11 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LKB1/STK11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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