LMO2 Antibody (1A9-3B11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-78626

Novus Biologicals, part of Bio-Techne
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NB110-78626
NB110-78626SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A9-3B11

Format

BSA Free

Concentration

1.0 mg/ml

View all LMO2 Antibodies »

Product Specifications

Immunogen

Recombinant human LMO2. [UniProt# P25791]

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34382935).

Localization

Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMO2 Antibody (1A9-3B11) - BSA Free

Western Blot: LMO2 Antibody (1A9-3B11)BSA Free [NB110-78626]

Western Blot: LMO2 Antibody (1A9-3B11)BSA Free [NB110-78626]

Western Blot: LMO2 Antibody (1A9-3B11) [NB110-78626] - Detection of LMO2 in Ramos cell lysate.
Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626]

Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626]

Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) [NB110-78626] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using LMO2 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear with some cytoplasmic staining was observed. Staining was performed by Histowiz.
Flow Cytometry: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626]

Flow Cytometry: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626]

Flow Cytometry: LMO2 Antibody (1A9-3B11) [NB110-78626] - An intracellular stain was performed on Ramos cells with LMO2 Antibody (1A9-3B11) NB110-78626 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
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Applications for LMO2 Antibody (1A9-3B11) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

1-2 ug/ml per million cells

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1:10-1:500

Western Blot

0.5 ug/ml
Application Notes
This LMO2 antibody is useful for Western blot, Immunoprecipitation, Immunofluorescence on tissue, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. In WB a band can be seen at ~24 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMO2

LMO2 (LIM-only protein 2) is a nuclear transcriptional regulator essential for normal blood cell development and it exerts its effects by mediating protein-protein interactions as well as by nucleating multicomponent transcriptional complexes. LMO2 interacts with LDB1 (LIM domain binding protein 1) through the tandem LIM domains of LMO2 and LID (LIM interaction domain) of LDB1. LMO2 also interacts with bHlH proteins TAL1/SCL, BEX2 and KDM5A, and form complex with TAL1/SCL. It functions with TAL1/SCL in the regulation of RBCs development and with LDB1 to maintain erythroid precursors in immature state. LMO2 has been associated with the maintenance of hematopoietic stem cells, RBCs differentiation, and angiogenesis in both normal development as well as during carcinogenesis. Disruption of LMO2 in mice causes death in midway through embryonic development as they do not develop RBCs. Excessive expression of LMO2 in T-cells, through either chromosomal translocations or retroviral insertion of gene therapy vectors, as well as transgenic overexpression of LMO2 in mouse model leads to the onset of T-cell acute lymphoblastic leukemia (T-ALL).

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

Additional LMO2 Products

Product Documents for LMO2 Antibody (1A9-3B11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LMO2 Antibody (1A9-3B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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