LMO2 Antibody (1A9-3B11) - BSA Free

Western Blot: LMO2 Antibody (1A9-3B11)BSA Free [NB110-78626]

Western Blot: LMO2 Antibody (1A9-3B11) [NB110-78626] - Detection of LMO2 in Ramos cell lysate.

Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626]

Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) [NB110-78626] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using LMO2 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear with some cytoplasmic staining was observed. Staining was performed by Histowiz.

Flow Cytometry: LMO2 Antibody (1A9-3B11) - BSA Free [NB110-78626]

Flow Cytometry: LMO2 Antibody (1A9-3B11) [NB110-78626] - An intracellular stain was performed on Ramos cells with LMO2 Antibody (1A9-3B11) NB110-78626 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).