LMO2 Antibody (LMO2/1971) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08268

Novus Biologicals, part of Bio-Techne
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NBP3-08268
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # LMO2/1971

Format

Azide and BSA Free

Concentration

1 mg/ml

View all LMO2 Antibodies »

Product Summary for LMO2 Antibody (LMO2/1971) - Azide and BSA Free

Immunogen

Recombinant human LMO2 protein fragment (around aa 23-140) (exact sequence is proprietary) (Uniprot: P25791)

Reactivity Notes

Others not known.

Localization

Nuclear, Cytoplasmic & Cell Surface

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07249).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for LMO2 Antibody (LMO2/1971) - Azide and BSA Free

Western Blot: LMO2 Antibody (LMO2/1971)Azide and BSA Free [NBP3-08268]

Western Blot: LMO2 Antibody (LMO2/1971)Azide and BSA Free [NBP3-08268]

Western Blot: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Western Blot Analysis of Ramos cell lysate using LMO2 Mouse Monoclonal Antibody (LMO2/1971).
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268]

Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268]

Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Formalin-fixed, paraffin-embedded human Pancreas stained with LMO2 Mouse Monoclonal Antibody (LMO2/1971).
Flow Cytometry: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268]

Flow Cytometry: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268]

Flow Cytometry: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Flow Cytometric Analysis of K562 cells using LMO2 Mouse Monoclonal Antibody (LMO2/1971) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red)
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Applications for LMO2 Antibody (LMO2/1971) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: LMO2

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

Additional LMO2 Products

Product Documents for LMO2 Antibody (LMO2/1971) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LMO2 Antibody (LMO2/1971) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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