Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LMO2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LMO2(NP_005565) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMO2 Antibody (OTI1G10) - Azide and BSA Free

Western Blot: LMO2 Antibody (OTI1G10)Azide and BSA Free [NBP2-71157]

Western Blot: LMO2 Antibody (OTI1G10)Azide and BSA Free [NBP2-71157]

Western Blot: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2.
Immunohistochemistry: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157]

Immunohistochemistry: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157]

Immunohistochemistry: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157]

Immunohistochemistry: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157]

Immunohistochemistry: LMO2 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71157] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for LMO2 Antibody (OTI1G10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMO2

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

Additional LMO2 Products

Product Documents for LMO2 Antibody (OTI1G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LMO2 Antibody (OTI1G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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