LMO2 Antibody (PD00-25)

Western Blot: LMO2 Antibody (PD00-25) [NBP3-32542]

Western Blot: LMO2 Antibody (PD00-25) [NBP3-32542] - Western blot analysis of LMO2 on K562 cell lysates with Rabbit anti-LMO2 antibody (NBP3-32542) at 1/1,000 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32542) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: LMO2 Antibody (PD00-25) [NBP3-32542]

Immunohistochemistry: LMO2 Antibody (PD00-25) [NBP3-32542] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LMO2 antibody (NBP3-32542) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32542) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.