LMO7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83915

Novus Biologicals, part of Bio-Techne
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NBP1-83915
NBP1-83915-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Porcine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LMO7 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEASYQSERVEEKGATY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%). Reactivity reported in scientific literature (PMID: 22253135). Porcine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LMO7 Antibody

Western Blot: LMO7 Antibody [NBP1-83915]

Western Blot: LMO7 Antibody [NBP1-83915]

Western Blot: LMO7 Antibody [NBP1-83915] - Analysis in human cell lines HeLa and HEK293 using anti-LMO7 antibody. Corresponding LMO7 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: LMO7 Antibody [NBP1-83915]

Immunocytochemistry/ Immunofluorescence: LMO7 Antibody [NBP1-83915]

Immunocytochemistry/Immunofluorescence: LMO7 Antibody [NBP1-83915] - Staining of human cell line A-431 shows localization to cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry: LMO7 Antibody [NBP1-83915]

Immunohistochemistry: LMO7 Antibody [NBP1-83915]

Immunohistochemistry: LMO7 Antibody [NBP1-83915] - LMO7 staining in Porcine Cardiac Tissue: No Primary Control, Isotype IgG Control, LMO7 1:500 dilution (Image 1 and 2). IHC images submitted by a verified customer review.
View 4 more images

Applications for LMO7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-83915 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMO7

LMO7 contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain. An F-box (FBX) domain is present in alternative splice variants. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. The encoded protein may be involved in protein-protein interactions. Multiple alternative splice variants have been described but their full-length sequences have not been determined.

Alternate Names

FBX20F-box only protein 20, KIAA0858FBXO20F-box protein Fbx20, LIM domain 7, LIM domain only 7, LIM domain only protein 7, LMO-7, LOMP, zinc-finger domain-containing protein

Gene Symbol

LMO7

Additional LMO7 Products

Product Documents for LMO7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LMO7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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