LMP7/PSMB8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33540

Novus Biologicals, part of Bio-Techne
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NBP1-33540
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%), Canine (91%), Porcine (94%), Sheep (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LMP7/PSMB8 Antibodies »

Product Summary for LMP7/PSMB8 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human LMP7/PSMB8. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Japanese medaka (80%), Xenopus laevis (83%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMP7/PSMB8 Antibody

Western Blot: LMP7/PSMB8 Antibody [NBP1-33540]

Western Blot: LMP7/PSMB8 Antibody [NBP1-33540]

Western Blot: LMP7/PSMB8 Antibody [NBP1-33540] - Sample 30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody [NBP1-33540]

Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody [NBP1-33540]

Immunocytochemistry/Immunofluorescence: LMP7/PSMB8 Antibody [NBP1-33540] - Analysis of paraformaldehyde-fixed A431, using Proteasome 20S LMP7 (NBP1-33540) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-33540]

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-33540]

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-33540] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
View 1 more images

Applications for LMP7/PSMB8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LMP7/PSMB8

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Long Name

Large Multifunctional Protease 7

Alternate Names

ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i, PSMB8, RING10

Gene Symbol

PSMB8

Additional LMP7/PSMB8 Products

Product Documents for LMP7/PSMB8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LMP7/PSMB8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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