LMP7/PSMB8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51957

Novus Biologicals, part of Bio-Techne
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NBP1-51957
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LMP7/PSMB8 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DVSDLLHQYREANQ corresponding to C-Terminus according to NP_683720.2.

Epitope

C-DVSDLLHQYREANQ

Specificity

This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LMP7/PSMB8 Antibody

Western Blot: LMP7/PSMB8 Antibody [NBP1-51957]

Western Blot: LMP7/PSMB8 Antibody [NBP1-51957]

Western Blot: LMP7/PSMB8 Antibody [NBP1-51957] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-51957]

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-51957]

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-51957] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for LMP7/PSMB8 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in human liver, lung and lymph node lysates is reported in scientific literature (PMID: 10878350).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMP7/PSMB8

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Long Name

Large Multifunctional Protease 7

Alternate Names

ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i, PSMB8, RING10

Entrez Gene IDs

5696 (Human); 16913 (Mouse); 24968 (Rat)

Gene Symbol

PSMB8

UniProt

NP_004150.1 (Human)

Additional LMP7/PSMB8 Products

Product Documents for LMP7/PSMB8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LMP7/PSMB8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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