LMW-PTP/ACP1 Antibody (PSH04-57)

Immunohistochemistry: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992]

Immunohistochemistry: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LMW-PTP/ACP1 antibody (NBP3-31992) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31992) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992]

Western Blot: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992] - Western blot analysis of LMW-PTP/ACP1 on different lysates with Rabbit anti-LMW-PTP/ACP1 antibody (NBP3-31992) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: COS-1 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 1 minute 33 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.